CRISPR-Cas9 is a reprogrammable DNA cutting machine that is being used to edit genomes in many organisms for research purposes. Its primary component, the Cas9 enzyme (orange), cuts genomic DNA ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Widely used as a genome editing tool, the CRISPR-Cas9 system allows researchers to precisely induce frameshift mutations in specific genes or insert foreign nucleic acid sequences into a cell’s DNA.

This new tool, known as minimal, versatile genetic perturbation technology (mvGPT), combines the powers of gene editing, ...

Analysis of CRISPR/Cas9 edited cells is done to check the efficacy of the system in introducing mutations (insertions, deletions, or substitutions) in the DNA sequences of the edited cell population.

With vasa-Cas9, high efficiency and self-limiting suppression was achieved. Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity.

Among his recent work at Drexel is the development of technologies based on the CRISPR-Cas9 system for genome mapping and sequencing, which have broad applications in molecular diagnostics, cell/gene ...

CRISPR-Cas9 editing leads to widespread loss of the targeted chromosome in human T cells, but scientists recently discovered a way to prevent such loss. To investigate how Cas9 gene editing affected T ...

Intellia Therapeutics, Inc. is a clinical stage genome editing company, which engages in the development of curative therapeutics using the CRISPR/Cas9 system. Its CRISPR/Cas9 system transforms ...