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9:51
YouTube
Catalyst University
Biotechniques | Basics of Making His-Tags & Nickel Affinity Chromatography
As always, the steps of putting a gene insert into vector/plasmid AND nickel affinity chromatography depend on your specific lab and protocol. Here, we look at the general theory of engineering a poly-His tagged protein and Ni affinity chromatography and what they are used for in the lab.
44.6K views
Mar 10, 2019
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